Phos-tag SDS-PAGE database EnvZ

OmpR

Member of the two-component regulatory system EnvZ/OmpR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates OmpR in response to environmental signals. Mw; 27,000

Image

In vitro autophosphorylation of OmpR in the presence of acetyl phosphate. Typical images of time-course autophosphorylation.

Related data

E.coli EnvZ in vitro kinase assay-1

E.coli EnvZ in vitro kinase assay-2

Reference	Kinoshita-Kikuta, E.,Kusamoto, H., Ono, S., Akayama, K., Eguchi, Y., Igarashi, M., Okajima, T., Utsumi,, R., Kinoshita, E., Koike, T. Quantitative monitoring of His and Asp phosphorylation in a bacterial signaling system by using Phos-tag Magenta/Cyan fluorescent dyes. *Electrophoresis*, 40,3005-3013 (2019) DOI 10.1002/elps.201900261
Compositionof gel	Zn²⁺–Phos-tag:　20µM Polyacrylamide: 10%(w/v)
Detection	CBB stain

Reference

Kinoshita-Kikuta, E.,Kusamoto, H., Ono, S., Akayama, K., Eguchi, Y., Igarashi, M., Okajima, T., Utsumi,, R., Kinoshita, E., Koike, T. Quantitative monitoring of His and Asp phosphorylation in a bacterial signaling system by using Phos-tag Magenta/Cyan fluorescent dyes. Electrophoresis, 40,3005-3013 (2019)

DOI 10.1002/elps.201900261

Compositionof gel

Zn²⁺–Phos-tag:　20µM

Polyacrylamide: 10%(w/v)

Detection

CBB stain